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KMID : 0370220180620010007
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Yakhak Hoeji
2018 Volume.62 No. 1 p.7 ~ p.13
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Rapid Screening of Sulfur Amino Acids in Human Plasma by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry for Diagnosis of Metabolic Disorder
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Thapa Maheshwor
Yoon Hye-Ran
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Abstract
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A rapid and simple screening method was developed for the simultaneous analysis of 6 sulfur-containing amino acids from human plasma at ng level. The procedure involves simple protein precipitation followed by direct analysis using UPLC-MS/MS. Calibration curves showed an excellent linear relationship with coefficients of determination (r2) of 0.9989-0.9998. LOD and LOQ were between 1-50 ng/mL and 10-100 ng/mL, respectively. When sulfur amino acids spiked into plasma sample, excellent linearity of detection response was demonstrated over the range between 10-10,000 ng/mL. This new analytical method is suitable for simultaneous screening of large number of samples in clinical application and metabolomics research.
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KEYWORD
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Sulfur containing amino acids, ultra-performance liquid chromatography-tandem mass spectrometry, metabolic disorders
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